8/13/2023 0 Comments Shelf life of klenow fragment![]() By introducing mutations in the gene that encodes Klenow, forms of the enzyme can be expressed that retain polymerase activity. In some situations, the 3 -> 5 exonuclease activity of Klenow fragment is either undesirable or not necessary. Wash buffer II (50 ml): Combine 48.375 ml of water, 1 ml of 5 M NaCl, 500. Klenow fragment is used frequently to prepare DNA that is labeled with radionuclides or other markers. This property is used to synthesize DNA complementary to single-stranded DNA templates. ![]() ![]() The addition of mononucleotides from dNTPs to the 3-OH terminus of DNA is catalyzed. It lacks the 53 exonuclease activity of the native enzyme. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED). Klenow fragment, including 10× reaction buffer (Thermo Fisher Scientific, cat. KLEN-RO) is a DNA-dependent 53 polymerase with 35 exonuclease activity. Life Technologies Inc., 5781 Van Allen Way, Carlsbad, California 92008. Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. B.00) Lot: Expiry Date: Store at -20 ☌ Components EP0421 EP0422 Klenow Fragment, exo. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. Product Overview Recommendations Documents FAQ Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I.
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